Femto-liter protein purification by parallel two-phase nanofluidics

Shu Matsuura, Yutaka Kazoe, Takehiko Kitamori

研究成果: Conference contribution

抄録

We succeeded in the formation of aqueous parallel two-phase flow in nanochannel for the first time and applied it to fL protein purification. As a result, the removal of 79% lipid and the remains of 91% protein were realized at two-phase contact time of 10 ms and the volume of 16 fL which was much smaller than the volume of single cell (pL). Therefore, this method will greatly contribute to development of pretreatment process in single cell protein analysis.

本文言語English
ホスト出版物のタイトル23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
出版社Chemical and Biological Microsystems Society
ページ618-619
ページ数2
ISBN(電子版)9781733419000
出版ステータスPublished - 2019
外部発表はい
イベント23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
継続期間: 2019 10月 272019 10月 31

出版物シリーズ

名前23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
国/地域Switzerland
CityBasel
Period19/10/2719/10/31

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 化学工学(その他)

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