Fiber optic light-scattering measurement system for evaluation of embryo viability: light-scattering characteristics from live mouse embryo

Harumi Itoh, Tsunenori M.D. Arai, Makoto Kikuchi

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We measured angular distribution of the light scattering from live mouse embryo with 632.8nm in wavelength to evaluate the embryo viability. We aim to measure the mitochondrial density in human embryo which have relation to the embryo viability. We have constructed the light scattering measurement system to detect the mitochondrial density non-invasively. We have employed two optical fibers for the illumination and sensing to change the angle between these fibers. There were two dips on the scattering angular distribution from the embryo. These dips existed on 30 and 85 deg. We calculated the scattering angular pattern by Mie theory to fit the measured scattering estimated scattering size and density. The best fitting was obtained when the particle size and density were 0.9 μm and 1010 particles per ml, respectively. These values coincided with the approximated values of mitochondrial in the embryo. The measured light scattering may mainly originated from mitochondria in spite of the existence of the various scattering particles in the embryo. Since our simple scattering measurement may offer the mitochondrial density in the embryo, it might become the practical method of human embryo on in vitro fertilization-embryo transfer.

本文言語English
ホスト出版物のタイトルProceedings of SPIE - The International Society for Optical Engineering
出版社Society of Photo-Optical Instrumentation Engineers
ページ108-114
ページ数7
ISBN(印刷版)0819423866
出版ステータスPublished - 1997 12 1
イベントLaser-Tissue Interaction VIII - San Jose, CA, USA
継続期間: 1997 2 91997 2 12

出版物シリーズ

名前Proceedings of SPIE - The International Society for Optical Engineering
2975
ISSN(印刷版)0277-786X

Other

OtherLaser-Tissue Interaction VIII
CitySan Jose, CA, USA
Period97/2/997/2/12

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • コンピュータ サイエンスの応用
  • 応用数学
  • 電子工学および電気工学

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