Fiber optic monitoring system for mouse embryo evaluation

Harumi Itoh, Tsunenori Arai, Makoto Kikuchi

研究成果: Conference contribution

抄録

We constructed a fiber-optic long-term monitoring system for mouse embryo, which developed a new quantitative and non-invasive evaluation method for human embryo. We monitored the changes of light scattering from mouse embryo which might relate to the state of blastomere and change in intercellular microstructures. We employed a couple of optical fibers to measure the scattering. These fibers were arranged in a light angle to the optical axis of a microscope to obtain adaptability for various measurements. A He-Ne laser with 632.8 nm in wavelength was used as a light source. The emission fiber (56 μmΦ; core) and sensing fiber (400 μmΦ; core, NA = 0.43) were used. We observed the light scattering changes from the embryo, which were over 200 times higher than the noise level with 30 s in period. This result might be attributed to the structural and/or functional changes in the mouse embryo.

本文言語English
ホスト出版物のタイトルProceedings of SPIE - The International Society for Optical Engineering
出版社Society of Photo-Optical Instrumentation Engineers
ページ458-461
ページ数4
ISBN(印刷版)0819417351
出版ステータスPublished - 1995 1 1
イベントAdvances in Fluorescence Sensing Technology II - San Jose, CA, USA
継続期間: 1995 2 61995 2 8

出版物シリーズ

名前Proceedings of SPIE - The International Society for Optical Engineering
2388
ISSN(印刷版)0277-786X

Other

OtherAdvances in Fluorescence Sensing Technology II
CitySan Jose, CA, USA
Period95/2/695/2/8

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • コンピュータ サイエンスの応用
  • 応用数学
  • 電子工学および電気工学

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