Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells

Patricia E. Burger, Sandra Coetzee, Wallace L. McKeehan, Mikio Kan, Perry Cook, Yong Fan, Toshio Suda, Robert P. Hebbel, Nicolas Novitzky, William A. Muller, E. Lynette Wilson

研究成果: Article査読

85 被引用数 (Scopus)

抄録

Recent experiments show that hematopoietic progenitor cell populations contain endothelial precursor cells. We have isolated a population of CD34+ cells that expresses fibroblast growth factor receptor-1 (FGFR-1) and that differentiates into endothelial cells in vitro. We find that 4.5% ± 2.1% of CD34+ cells isolated from bone marrow, cord blood, and mobilized peripheral blood express FGFR-1 and that viable CD34+FGFR+ cells are small, with little granularity, and express both primitive hematopoietic and endothelial markers on their surface. The primitive hematopoietic markers AC133, c-kit, and Thy-1 are coexpressed by 75%, 85%, and 64% of CD34+FGFR+ cells, respectively. Most of the CD34+FGFR+ cells also express antigens found on endothelial cells, such as CD31, vascular endothelial growth factor receptor-2, and the endothelial-specific cell surface marker, vascular endothelial cadherin (VE-cadherin), whereas 56% to 60% of the cells express Tie, Tek, and the endothelial-specific marker, P1H12. The CD34+FGFR+ population is enriched in cells expressing endothelial-specific antigens compared with the CD34+ population. Isolated CD34+FGFR+ cells grow slowly in culture, are stimulated by fibroblast growth factor-2 and vascular endothelial growth factor, and give rise to cells that express von Willebrand factor and VE-cadherin and that incorporate acetylated low-density lipoprotein. These experiments show that FGFR-1 is expressed by a subpopulation of CD34+ cells that give rise to endothelial cells in vitro, indicating that this population contains endothelial stem/progenitor cells.

本文言語English
ページ(範囲)3527-3535
ページ数9
ジャーナルBlood
100
10
DOI
出版ステータスPublished - 2002 11 15
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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