TY - JOUR
T1 - Fine-tuning in mineral cross-linking of biopolymer nanoparticle for incorporation and release of cargo
AU - Fukui, Yuuka
AU - Kabayama, Narumi
AU - Fujimoto, Keiji
PY - 2015/12/1
Y1 - 2015/12/1
N2 - We developed a mineral cross-linking strategy to prepare a biopolymer-based nanoparticle using calcium phosphate (CaP) as a cross-linker. Nanoparticles were first formed by mixing deoxyribonucleic acid (DNA) with cationic surfactants, and were cross-linked by CaP precipitation. After removal of the surfactants, we carried out the alternative dialysis of nanoparticles against CaCl2 aqueous solution and phosphate buffered solution for further mineral cross-linking. XRD and FT-IR studies revealed that the resultant nanoparticles were produced by mineral cross-linkages of hydroxyapatite (HAp) and the crystal amount and properties such as morphology and crystallinity could be well-controlled by the reaction conditions. Chemical dyes could be incorporated into nanoparticles via their affinities with crystal faces of HAp and DNA. Their release was tunable by crystal amount and properties of mineral cross-linkages. Also, the release could be triggered by mineral dissolution in response to pH. Such a mineral cross-linking will open up a potential way to provide a nanoparticle with versatile functions such as cleavable cross-linking, binding affinity for cargos, and pH-responsive release.
AB - We developed a mineral cross-linking strategy to prepare a biopolymer-based nanoparticle using calcium phosphate (CaP) as a cross-linker. Nanoparticles were first formed by mixing deoxyribonucleic acid (DNA) with cationic surfactants, and were cross-linked by CaP precipitation. After removal of the surfactants, we carried out the alternative dialysis of nanoparticles against CaCl2 aqueous solution and phosphate buffered solution for further mineral cross-linking. XRD and FT-IR studies revealed that the resultant nanoparticles were produced by mineral cross-linkages of hydroxyapatite (HAp) and the crystal amount and properties such as morphology and crystallinity could be well-controlled by the reaction conditions. Chemical dyes could be incorporated into nanoparticles via their affinities with crystal faces of HAp and DNA. Their release was tunable by crystal amount and properties of mineral cross-linkages. Also, the release could be triggered by mineral dissolution in response to pH. Such a mineral cross-linking will open up a potential way to provide a nanoparticle with versatile functions such as cleavable cross-linking, binding affinity for cargos, and pH-responsive release.
KW - Alternative dialysis
KW - Calcium phosphate
KW - Cross-linking
KW - DNA
KW - Mineralization
KW - Nanoparticle
KW - Release control
UR - http://www.scopus.com/inward/record.url?scp=84941921878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941921878&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2015.09.011
DO - 10.1016/j.colsurfb.2015.09.011
M3 - Article
C2 - 26387068
AN - SCOPUS:84941921878
VL - 136
SP - 168
EP - 174
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
M1 - 7351
ER -