Flexible Accelerated STOP Tetracycline Operator-Knockin (FAST): A Versatile and Efficient New Gene Modulating System

Kenji F. Tanaka, Susanne E. Ahmari, E. David Leonardo, Jesse W. Richardson-Jones, Elaine C. Budreck, Peter Scheiffele, Shouta Sugio, Naoko Inamura, Kazuhiro Ikenaka, René Hen

研究成果: Article査読

69 被引用数 (Scopus)

抄録

We created the Flexible Accelerated STOP Tetracycline Operator (tetO)-knockin (FAST) system, an efficient method for manipulating gene expression in vivo to rapidly screen animal models of disease. A single gene targeting event yields two distinct knockin mice-STOP-tetO and tetO knockin-that permit generation of multiple strains with variable expression patterns: 1) knockout, 2) Cre-mediated rescue, 3) tetracycline-controlled transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled transcriptional activator (tTA)-mediated overexpression, and 5) tetracycline-controlled transcriptional silencer (tTS)-mediated conditional knockout/knockdown. Using the FAST system, multiple gain-of-function and loss-of-function strains can therefore be generated on a time scale not previously achievable. These strains can then be screened for clinically relevant abnormalities. We demonstrate the flexibility and broad applicability of the FAST system by targeting several genes encoding proteins implicated in neuropsychiatric disorders: Mlc1, neuroligin 3, the serotonin 1A receptor, and the serotonin 1B receptor.

本文言語English
ページ(範囲)770-773
ページ数4
ジャーナルBiological Psychiatry
67
8
DOI
出版ステータスPublished - 2010 4 15
外部発表はい

ASJC Scopus subject areas

  • Biological Psychiatry

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