Flow-through type calcium ion selective optodes based on novel neutral ionophores and a lipophilic anionic dye

Hideaki Hisamoto, Kazuhiko Watanabe, Eriko Nakagawa, Dwi Siswanta, Yushi Shichi, Koji Suzuki

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Flow-through type calcium ion selective optodes based on novel neutral ionophores and a lipophilic anionic dye were developed. The novel calcium ion selective neutral ionophores designed and synthesized were double-armed diazacrown ether compounds possessing glycolic amides as the binding arms. Cation sensing material was prepared by coating pellicular-type ODS (octadecylsilica) beads with a lipophilic organic liquid which incorporates a novel neutral calcium ionophore and a lipophilic anionic dye of the diphenyl amine type. These beads were packed in a flow-through optical cell, and the Ca2+ measurement was performed to measure the absorbance change of the sensing beads at 516 nm. The best optode tested exhibited a good response to calcium ions in the concentration range from 10-7 M to 10-1 M with 0.05 M Tris-HCl of pH 7.0 as the flowing buffer solution. This optode showed excellent Ca2+ selectivity of over 10-4 in selectivity coefficient values (ki,jopt.; i = primary ion (Ca2+); j = interfering ion) against Na+, K+ and Mg2+ which are primary interferents for human serum analysis. Sensor response theory, response time, selectivity, and application to the determination of calcium ion concentration in human serum were also discussed together with experimental results.

本文言語English
ページ(範囲)179-187
ページ数9
ジャーナルAnalytica Chimica Acta
299
2
DOI
出版ステータスPublished - 1994 12 30

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 環境化学
  • 分光学

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