Flt1 and Flk1 mediate regulation of intraocular pressure and their double heterozygosity causes the buphthalmia in mice

Keigo Sano, Osamu Katsuta, Satoshi Shirae, Yoshiaki Kubota, Masatsugu Ema, Toshio Suda, Masatsugu Nakamura, Masanori Hirashima

研究成果: Article

4 被引用数 (Scopus)

抄録

Flt1 and Flk1 are receptor tyrosine kinases for vascular endothelial growth factor-A which play a crucial role in physiological and pathological angiogenesis. To study genetic interaction between the Flt1 and Flk1 genes, we crossed between Flt1 and Flk1 heterozygous (Flt1+/- and Flk1+/-) mice. We found that Flt1; Flk1 double heterozygous (Flt1+/-; Flk1+/-) mice showed enlarged eyes similar to the buphthalmia detected in human congenital glaucoma with elevation of intraocular pressure (IOP). Actually, IOP was elevated in Flt1+/-; Flk1+/- mice and also in Flt1 or Flk1 single heterozygous mice. However, none of these mutants showed hallmarks of glaucoma such as ganglion cell death and excavation of optic disc. To clarify the pathological causes for enlarged eyes and elevated IOP, we investigate the mice from matings between Flt1+/- and Flk1+/- mice. Flt1+/- mice showed enlarged Schlemm's canal and disordered collagen fibers in the sclera, whereas Flk1+/- mice showed atrophied choriocapillaris in the choroid. These tissues are a part of the main outflow and alternative uveoscleral outflow pathway of the aqueous humor, suggesting that these pathological changes found in Flt1+/- and Flk1+/- mice are associated with the buphthalmia in Flt1+/-; Flk1+/- mice.

本文言語English
ページ(範囲)422-427
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
420
2
DOI
出版ステータスPublished - 2012 4 6

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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