Frequent spontaneous seizures followed by spatial working memory/anxiety deficits in mice lacking sphingosine 1-phosphate receptor 2

Noriyuki Akahoshi, Yasuki Ishizaki, Hiroki Yasuda, Yoshiya L. Murashima, Toshikazu Shinba, Kaoru Goto, Toshiyuki Himi, Jerold Chun, Isao Ishii

研究成果: Article査読

30 被引用数 (Scopus)

抄録

The diverse physiological effects of sphingosine 1-phosphate (S1P) are mostly mediated by its five cognate G protein-coupled receptors, S1P 1-S1P 5, which have attracted much attention as future drug targets. To gain insight into S1P 2-mediated signaling, we analyzed frequent spontaneous seizures in S1P 2-deficient (S1P 2 -/-) mice obtained after several backcrosses onto a C57BL/6N background. Full-time video recording of 120 S1P 2 -/- mice identified 420 seizures both day and night between postnatal days 25 and 45, which were accompanied by high-voltage synchronized cortical discharges and a series of typical episodes: wild run, tonic-clonic convulsion, freezing, and, occasionally, death. Nearly 40% of 224 S1P 2 -/- mice died after such seizures, while the remaining 60% of the mice survived to adulthood; however, approximately half of the deliveries from S1P 2 -/- pregnant mice resulted in neonatal death. In situ hybridization revealed exclusive s1p 2 expression in the hippocampal pyramidal/granular neurons of wild-type mice, and immunohistochemistry/microarray analyses identified enhanced gliosis in the whole hippocampus and its neighboring neocortex in seizure-prone adult S1P 2 -/- mice. Seizure-prone adult S1P 2 -/- mice displayed impaired spatial working memory in the eight-arm radial maze test and increased anxiety in the elevated plus maze test, whereas their passive avoidance learning memory performance in the step-through test and hippocampal long-term potentiation was indistinguishable from that of wild-type mice. Our findings suggest that blockade of S1P 2 signaling may cause seizures/hippocampal insults and impair some specific central nervous system functions.

本文言語English
ページ(範囲)659-665
ページ数7
ジャーナルEpilepsy and Behavior
22
4
DOI
出版ステータスPublished - 2011 12月
外部発表はい

ASJC Scopus subject areas

  • 神経学
  • 臨床神経学
  • 行動神経科学

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