Function and regulation of taurine transport at the inner blood-retinal barrier

Masatoshi Tomi, Tomoyuki Terayama, Tomoyuki Isobe, Fuminobu Egami, Akihisa Morito, Michio Kurachi, Sumio Ohtsuki, Young Sook Kang, Tetsuya Terasaki, Ken ichi Hosoya

研究成果: Article

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In the retina, taurine exerts a number of neuroprotective functions as an osmolyte and antioxidant. The purpose of the present study was to elucidate the taurine transport system(s) at the inner blood-retinal barrier (BRB). [3H]Taurine transport at the inner BRB was characterized using in vivo integration plot analysis and a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB2 cells). The expression of the taurine transporter (TauT) was demonstrated by RT-PCR and immunoblot analyses. The apparent influx permeability clearance of [3H]taurine in the rat retina was found to be 259 μL/(min{radical dot}g retina), supporting carrier-mediated influx transport of taurine at the BRB. [3H]Taurine uptake by TR-iBRB2 cells was Na+-, Cl-- and concentration-dependent with a Km of 22.2 μM and inhibited by TauT inhibitors, such as β-alanine and hypotaurine. RT-PCR and immunoblot analyses demonstrated that TauT is expressed in TR-iBRB2 and primary cultured human retinal endothelial cells. The uptake of [3H]taurine and the expression of TauT mRNA in TR-iBRB2 cells increased under hypertonic conditions but decreased following pretreatment with excess taurine. In conclusion, TauT most likely mediates taurine transport and regulate taurine transport at the inner BRB.

元の言語English
ページ(範囲)100-106
ページ数7
ジャーナルMicrovascular Research
73
発行部数2
DOI
出版物ステータスPublished - 2007 3

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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    Tomi, M., Terayama, T., Isobe, T., Egami, F., Morito, A., Kurachi, M., Ohtsuki, S., Kang, Y. S., Terasaki, T., & Hosoya, K. I. (2007). Function and regulation of taurine transport at the inner blood-retinal barrier. Microvascular Research, 73(2), 100-106. https://doi.org/10.1016/j.mvr.2006.10.003