Functional analysis of GS28, an intra-Golgi SNARE, in Caenorhabditis elegans

Masashi Maekawa, Takao Inoue, Hiroyuki Kobuna, Taki Nishimura, Keiko Gengyo-Ando, Shohei Mitani, Hiroyuki Arai

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Intra-Golgi retrograde transport is assumed to maintain Golgi function by recycling Golgi-resident proteins to younger cisternae in the progression of entire Golgi stack from cis to trans. GS28 (Golgi SNARE of 28kDa, also known as GOS28) is a Golgi-localized SNARE protein and has been implicated in intra-Golgi retrograde transport. However, the in vivo functions of GS28, and consequently, the roles of the intra-Golgi retrograde transport in animal development are largely unknown. In this study, we generated deletion mutants of Caenorhabditis elegans GS28 and performed a synthetic lethal RNAi screen using GS28 mutants. We found that another Golgi-localized SNARE, Ykt6, functions cooperatively with GS28 in embryonic development. During post-embryonic development, GS28 mutants exhibited reduced seam cell numbers and a missing ray phenotype under Ykt6 knockdown conditions, suggesting that cell proliferation and/ or differentiation of stem cell-like seam cells are impaired in GS28- and Ykt6-depleted worms. We also demonstrated that GS28 and Ykt6 act redundantly for the proper expression of Golgi-resident proteins in adult intestinal cells. This study reveals the in vivo importance of the Golgi-localized SNAREs GS28 and Ykt6.

本文言語English
ページ(範囲)1003-1013
ページ数11
ジャーナルGenes to Cells
14
8
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 細胞生物学

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