Functional and morphological analysis of rehydrated lyophilized human platelets

T. A. Takahashi, K. Nakai, N. Sato, S. Fujikawa, K. Tadokoro, T. Jyuji, Mitsuru Murata, Y. Ikeda

研究成果: Article

抄録

We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

元の言語English
ページ(範囲)637-640
ページ数4
ジャーナルJapanese Journal of Artificial Organs
26
発行部数3
出版物ステータスPublished - 1997

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Blood Platelets
Ristocetin
Integrin beta3
P-Selectin
Freeze Drying
Membrane Glycoproteins
Platelet Activation
Bovine Serum Albumin
Collagen
Electrons

ASJC Scopus subject areas

  • Biophysics

これを引用

Takahashi, T. A., Nakai, K., Sato, N., Fujikawa, S., Tadokoro, K., Jyuji, T., ... Ikeda, Y. (1997). Functional and morphological analysis of rehydrated lyophilized human platelets. Japanese Journal of Artificial Organs, 26(3), 637-640.

Functional and morphological analysis of rehydrated lyophilized human platelets. / Takahashi, T. A.; Nakai, K.; Sato, N.; Fujikawa, S.; Tadokoro, K.; Jyuji, T.; Murata, Mitsuru; Ikeda, Y.

:: Japanese Journal of Artificial Organs, 巻 26, 番号 3, 1997, p. 637-640.

研究成果: Article

Takahashi, TA, Nakai, K, Sato, N, Fujikawa, S, Tadokoro, K, Jyuji, T, Murata, M & Ikeda, Y 1997, 'Functional and morphological analysis of rehydrated lyophilized human platelets', Japanese Journal of Artificial Organs, 巻. 26, 番号 3, pp. 637-640.
Takahashi TA, Nakai K, Sato N, Fujikawa S, Tadokoro K, Jyuji T その他. Functional and morphological analysis of rehydrated lyophilized human platelets. Japanese Journal of Artificial Organs. 1997;26(3):637-640.
Takahashi, T. A. ; Nakai, K. ; Sato, N. ; Fujikawa, S. ; Tadokoro, K. ; Jyuji, T. ; Murata, Mitsuru ; Ikeda, Y. / Functional and morphological analysis of rehydrated lyophilized human platelets. :: Japanese Journal of Artificial Organs. 1997 ; 巻 26, 番号 3. pp. 637-640.
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AU - Takahashi, T. A.

AU - Nakai, K.

AU - Sato, N.

AU - Fujikawa, S.

AU - Tadokoro, K.

AU - Jyuji, T.

AU - Murata, Mitsuru

AU - Ikeda, Y.

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N2 - We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

AB - We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

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