Functional and morphological analysis of rehydrated lyophilized human platelets

T. A. Takahashi, K. Nakai, N. Sato, S. Fujikawa, K. Tadokoro, T. Jyuji, M. Murata, Y. Ikeda

研究成果: Article査読

抄録

We have prepared rehydrated lyophilized (RL) human platelets as a candidate for platelet substitute. Platelets prepared from buffy-coats were fixed with 1.8% paraformaldehyde, frozen in the presence of 5% bovine serum albumin and stored after lyophilization. RL platelets preserved ristocetin- dependent aggregation buy not collagen-induced aggregation. Flow cytometric analysis of membrane glycoproteins revealed the presence of GPIb and GPIIIa. This also showed appearance of GMP-140, which is a marker of platelet activation. Transmission electron micrographic analysis showed no morphological difference between fresh and RL platelets. These results suggest that RL platelets is a promissing candidate that can substitute the adhesion capacity of platelets.

本文言語English
ページ(範囲)637-640
ページ数4
ジャーナルJapanese Journal of Artificial Organs
26
3
出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 生物理学

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