Functional cloning and characterization of a multidrug efflux pump, MexHI-OpmD, from a Pseudomonas aeruginosa mutant

Hiroshi Sekiya, Takehiko Mima, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article

48 引用 (Scopus)

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We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.

元の言語English
ページ(範囲)2990-2992
ページ数3
ジャーナルAntimicrobial Agents and Chemotherapy
47
発行部数9
DOI
出版物ステータスPublished - 2003 9 1

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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