抄録
We examined the development of cholinergic neuronal functions and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) responses in cultured embryonic rat septal cells. Choline acetyltransferase activity was increased from 4 to 6 days in culture and reached a plateau at day 8. Acetylcholine release was increased from 6 to 8 days in culture. AMPA-induced increase in intracellular Ca2+ level was observed at 3 days in culture and most of the AMPA-responsive cells coincided with high-K+ responsive cells. These results suggest that cholinergic neurons develop their neuronal functions about 8 days under cultured conditions, and functional expression of AMPA receptors precedes the cholinergic functional development.
本文言語 | English |
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ページ(範囲) | 89-92 |
ページ数 | 4 |
ジャーナル | Neuroscience Letters |
巻 | 311 |
号 | 2 |
DOI | |
出版ステータス | Published - 2001 9月 28 |
ASJC Scopus subject areas
- 神経科学(全般)