Functional expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors precedes the development of cholinergic phenotype in embryonic rat septal cells in culture

Takeshi Suzuki, Takeshi Matsugi, Ryoichi Takagi, Masaru Kanagawa, Mihoko Hirata, Takeshi Nakamura, Yoshihisa Kudo, Koichiro Kawashima

研究成果: Article

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We examined the development of cholinergic neuronal functions and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) responses in cultured embryonic rat septal cells. Choline acetyltransferase activity was increased from 4 to 6 days in culture and reached a plateau at day 8. Acetylcholine release was increased from 6 to 8 days in culture. AMPA-induced increase in intracellular Ca2+ level was observed at 3 days in culture and most of the AMPA-responsive cells coincided with high-K+ responsive cells. These results suggest that cholinergic neurons develop their neuronal functions about 8 days under cultured conditions, and functional expression of AMPA receptors precedes the cholinergic functional development.

元の言語English
ページ(範囲)89-92
ページ数4
ジャーナルNeuroscience Letters
311
発行部数2
DOI
出版物ステータスPublished - 2001 9 28

ASJC Scopus subject areas

  • Neuroscience(all)

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