Functional expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors precedes the development of cholinergic phenotype in embryonic rat septal cells in culture

Takeshi Suzuki, Takeshi Matsugi, Ryoichi Takagi, Masaru Kanagawa, Mihoko Hirata, Takeshi Nakamura, Yoshihisa Kudo, Koichiro Kawashima

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We examined the development of cholinergic neuronal functions and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) responses in cultured embryonic rat septal cells. Choline acetyltransferase activity was increased from 4 to 6 days in culture and reached a plateau at day 8. Acetylcholine release was increased from 6 to 8 days in culture. AMPA-induced increase in intracellular Ca2+ level was observed at 3 days in culture and most of the AMPA-responsive cells coincided with high-K+ responsive cells. These results suggest that cholinergic neurons develop their neuronal functions about 8 days under cultured conditions, and functional expression of AMPA receptors precedes the cholinergic functional development.

本文言語English
ページ(範囲)89-92
ページ数4
ジャーナルNeuroscience Letters
311
2
DOI
出版ステータスPublished - 2001 9月 28

ASJC Scopus subject areas

  • 神経科学(全般)

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