@article{c2f7c3f0c36047cd8b67a27678577b25,
title = "Functional Neurons Generated from T Cell-Derived Induced Pluripotent Stem Cells for Neurological Disease Modeling",
abstract = "Modeling of neurological diseases using induced pluripotent stem cells (iPSCs) derived from the somatic cells of patients has provided a means of elucidating pathogenic mechanisms and performing drug screening. T cells are an ideal source of patient-specific iPSCs because they can be easily obtained from samples. Recent studies indicated that iPSCs retain an epigenetic memory relating to their cell of origin that restricts their differentiation potential. The classical method of differentiation via embryoid body formation was not suitable for T cell-derived iPSCs (TiPSCs). We developed a neurosphere-based robust differentiation protocol, which enabled TiPSCs to differentiate into functional neurons, despite differences in global gene expression between TiPSCs and adult human dermal fibroblast-derived iPSCs. Furthermore, neurons derived from TiPSCs generated from a juvenile patient with Parkinson's disease exhibited several Parkinson's disease phenotypes. Therefore, we conclude that TiPSCs are a useful tool for modeling neurological diseases.",
author = "Takuya Matsumoto and Koki Fujimori and Tomoko Andoh-Noda and Takayuki Ando and Naoko Kuzumaki and Manabu Toyoshima and Hirobumi Tada and Kent Imaizumi and Mitsuru Ishikawa and Ryo Yamaguchi and Miho Isoda and Zhi Zhou and Shigeto Sato and Tetsuro Kobayashi and Manami Ohtaka and Ken Nishimura and Hiroshi Kurosawa and Takeo Yoshikawa and Takuya Takahashi and Mahito Nakanishi and Manabu Ohyama and Nobutaka Hattori and Wado Akamatsu and Hideyuki Okano",
note = "Funding Information: The authors thank Dr. Hajime Komano and all the members of H.O.{\textquoteright}s laboratory for their encouragement and support. This work was supported by funding from the Project for the Realization of Regenerative Medicine and Support for Core Institutes for iPS Cell Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to H.O., Research Center Network for Realization Research Centers/Projects of Regenerative Medicine (the Program for Intractable Disease Research utilizing disease-specific iPS Cells) from the Japan Science and Technology Agency (JST) and Japan Agency for Medical Research and Development (AMED) to H.O., the New Energy and Industrial Technology Development Organization (NEDO) to H.O. and W.A., the Japan Society for the Promotion of Science (JSPS) to W.A., and a Grant-in-Aid for the Global COE Program from MEXT to Keio University. H.O. is a scientific consultant for SanBio, Co. Ltd., Eisai, Co., Ltd. Publisher Copyright: {\textcopyright} 2016 The Authors.",
year = "2016",
month = mar,
day = "8",
doi = "10.1016/j.stemcr.2016.01.010",
language = "English",
volume = "6",
pages = "422--435",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "3",
}
