Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa

Takehiko Mima, Hiroshi Sekiya, Tohru Mizushima, Teruo Kuroda, Tomofusa Tsuchiya

研究成果: Article査読

46 被引用数 (Scopus)

抄録

We cloned two operons for putative RND-type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(-opmE) and in another operon mexMN. Introduction of the mexPQ-opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ-OpmE and MexMN-OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.

本文言語English
ページ(範囲)999-1002
ページ数4
ジャーナルMicrobiology and Immunology
49
11
DOI
出版ステータスPublished - 2005

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • ウイルス学

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