Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity

Tetsuya S. Tanaka, Tilo Kunath, Wendy L. Kimber, Saied A. Jaradat, Carole A. Stagg, Masayuki Usuda, Takashi Yokota, Hitoshi Niwa, Janet Rossant, Minoru S.H. Ko

研究成果: Review article査読

193 被引用数 (Scopus)

抄録

Large-scale gene expression profiling was performed on embryo-derived stem cell lines to identify molecular signatures of pluripotency and lineage specificity. Analysis of pluripotent embryonic stem (ES) cells, extraembryonic-restricted trophoblast stem (TS) cells, and terminally-differentiated mouse embryo fibroblast (MEF) cells identified expression profiles unique to each cell type, as well as genes common only to ES and TS cells. Whereas most of the MEF-specific genes had been characterized previously, the majority (67%) of the ES-specific genes were novel and did not include known differentiated cell markers. Comparison with microarray data from embryonic material demonstrated that ES-specific genes were underrepresented in all stages sampled, whereas TS-specific genes included known placental markers. Investigation of four novel TS-specific genes showed trophoblast-restricted expression in cell lines and in vivo, whereas one uncharacterized ES-specific gene, Esg-1, was found to be exclusively associated with pluripotency. We suggest that pluripotency requires a set of genes not expressed in other cell types, whereas lineage-restricted stem cells, like TS cells, express genes predictive of their differentiated lineage.

本文言語English
ページ(範囲)1921-1928
ページ数8
ジャーナルGenome Research
12
12
DOI
出版ステータスPublished - 2002 12 1
外部発表はい

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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