Gene expression signature and the prediction of ulcerative colitis-associated colorectal cancer by DNA microarray

Toshiaki Watanabe, Takashi Kobunai, Etsuko Toda, Takamitsu Kanazawa, Yoshihiro Kazama, Junichiro Tanaka, Toshiaki Tanaka, Yoko Yamamoto, Keisuke Hata, Tetsu Kojima, Tadashi Yokoyama, Tsuyoshi Konishi, Yoshihiro Okayama, Yoshikazu Sugimoto, Toshinori Oka, Shin Sasaki, Yohichi Ajioka, Tetsuichiro Muto, Hirokazu Nagawa

研究成果: Article査読

51 被引用数 (Scopus)

抄録

Purpose: Ulcerative colitis (UC) is associated with a high risk of colorectal cancer. To identify genes that could predict the development of cancer in UC, we conducted a DNA microarray analysis using nonneoplastic rectal mucosa of UC patients. Experimental Design: Gene expression in nonneoplastic mucosa of 53 UC patients were examined. Gene expression profiles were examined using human Genome U133 Plus 2.0 gene chip array (Affymetrix). Among 53 UC patients, 10 had UC-associated cancer (UC-Ca group) whereas 43 did not (UC-NonCa group). Results: By comparing gene expression profiles of nonneoplastic rectal mucosae between the UC-Ca and UC-NonCa groups, we could identify 40 genes that were differentially expressed between two groups. The list of discriminating genes included low-density lipoprotein receptor-related protein (LRP5 and LRP6). Previous studies suggested that LRP5 and LRP6 expression promotes cancer cell proliferation and tumorigenesis and are considered as candidate oncogenes. In the present study, both LRP5 and LRP6 showed significantly higher expression in the UC-Ca group, which suggests the importance of these genes in the development of UC-associated colorectal cancers. With the 40 selected discriminating genes, we did class prediction of the development of colorectal neoplasms in UC patients. Using the k-nearest neighbor method and the support vector machine, we could predict the development of UC-associated neoplasms with an accuracy of 86.8% and 98.1%, respectively. Conclusions: These findings have important implications for the early detection of malignant lesions in UC and may provide directions for future research into the molecular mechanisms of UC-associated cancer.

本文言語English
ページ(範囲)415-420
ページ数6
ジャーナルClinical Cancer Research
13
2 I
DOI
出版ステータスPublished - 2007 1 15
外部発表はい

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

フィンガープリント 「Gene expression signature and the prediction of ulcerative colitis-associated colorectal cancer by DNA microarray」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル