Gene transfer and analysis of the gene expression mechanism using pDNA/chitosan complexes coated with lactose-modified PEG derivatives

Rieko Okan, Kohei Koya, Yoshiyuki Koyama, Toshinori Sato

研究成果: Paper査読

抄録

Chitosan is one of naturally occurring polycations and is useful as a non-viral vector for gene delivery into cells. As PEG-C is carboxylic acid-pendant poly(ethylene glycol) derivatives, the negatively charged PEG-C can coat the surface of positively charged plasmid/chitosan complexes. The pDNA/chitosan complexes coated with the lactose modified PEG-C showed the high gene expression. To analyze the cell transfection mechanism, the amount of cell uptake was determined using a FACS, and intracellular transfer of the complexes was observed using a confocal laser scanning microscope (CLSM). The amount of cell uptake did not correlate with the gene expression, but the cell uptake of lactose-bearing complexes is faster than conventional PEG-C complexes. The CSLM showed that the pDNA/chitosan/PEG-C and pDNA/chitosan/Lac-PEG-C complexes were taken up into cells without dissociation. Moreover, the pDNA/chitosan and pDNA/chitosan/Lac-PEG-C complexes showed nuclear import after eight hours.

本文言語English
ページ数1
出版ステータスPublished - 2005 12 1
イベント54th SPSJ Annual Meeting 2005 - Yokohama, Japan
継続期間: 2005 5 252005 5 27

Other

Other54th SPSJ Annual Meeting 2005
国/地域Japan
CityYokohama
Period05/5/2505/5/27

ASJC Scopus subject areas

  • 工学(全般)

フィンガープリント

「Gene transfer and analysis of the gene expression mechanism using pDNA/chitosan complexes coated with lactose-modified PEG derivatives」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル