Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.
|出版ステータス||Published - 2005 12 1|
|イベント||54th SPSJ Annual Meeting 2005 - Yokohama, Japan|
継続期間: 2005 5 25 → 2005 5 27
|Other||54th SPSJ Annual Meeting 2005|
|Period||05/5/25 → 05/5/27|
ASJC Scopus subject areas