TY - JOUR
T1 - Generation and validation of a common marmoset embryonic stem cell line ActiCre-B1 that ubiquitously expresses a tamoxifen-inducible Cre-driver
AU - Yoshimatsu, Sho
AU - Ohtsu, Kanae
AU - Sato, Tsukika
AU - Yamamoto, Masafumi
AU - Sasaki, Erika
AU - Shiozawa, Seiji
AU - Okano, Hideyuki
N1 - Funding Information:
We would like to express our thanks to Ms. Fumiko Ozawa (Keio University) for assisting in this study. We also thank all the laboratory members of H.O. for their encouragement and generous support. Data presented herein were obtained in the “Construction of System for Spread of Primate Model Animals” project, performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP20dm0207001 to H.O.), and Scientific Research in Innovative Areas, a MEXT Grant-in-Aid project FY2014-2018 “Brain Protein Aging and Dementia Control” (Grant ID: 26117007 to H.O.). This study was also supported by JSPS KAKENHI Grant Number 19 J12871 and 20 K22660 (to S.Y), RIKEN Junior Research Associate Program (to S.Y.) and internal budgets from Keio University including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.).
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/3
Y1 - 2021/3
N2 - We previously reported the efficient targeted introduction of transgenes into the genomic DNA of the common marmoset (Callithrix jacchus) using CRISPR-Cas9. In this study, we generated a marmoset embryonic stem cell (ESC) line that ubiquitously expresses the tamoxifen-inducible Cre-driver ERT2CreERT2. We validated the pluripotency of the ESC line and also successfully demonstrated the temporal control of the Cre-driver in a tamoxifen-dependent manner in the ESCs. This ESC line, named ActiCre-B1, will be a valuable resource for in vitro investigation of phenotypes related to embryonic lethality by targeted knockout of functionally important genes.
AB - We previously reported the efficient targeted introduction of transgenes into the genomic DNA of the common marmoset (Callithrix jacchus) using CRISPR-Cas9. In this study, we generated a marmoset embryonic stem cell (ESC) line that ubiquitously expresses the tamoxifen-inducible Cre-driver ERT2CreERT2. We validated the pluripotency of the ESC line and also successfully demonstrated the temporal control of the Cre-driver in a tamoxifen-dependent manner in the ESCs. This ESC line, named ActiCre-B1, will be a valuable resource for in vitro investigation of phenotypes related to embryonic lethality by targeted knockout of functionally important genes.
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U2 - 10.1016/j.scr.2021.102164
DO - 10.1016/j.scr.2021.102164
M3 - Article
C2 - 33453576
AN - SCOPUS:85099234847
SN - 1873-5061
VL - 51
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 102164
ER -