Generation and validation of a common marmoset embryonic stem cell line ActiCre-B1 that ubiquitously expresses a tamoxifen-inducible Cre-driver

Sho Yoshimatsu, Kanae Ohtsu, Tsukika Sato, Masafumi Yamamoto, Erika Sasaki, Seiji Shiozawa, Hideyuki Okano

研究成果: Article査読

抄録

We previously reported the efficient targeted introduction of transgenes into the genomic DNA of the common marmoset (Callithrix jacchus) using CRISPR-Cas9. In this study, we generated a marmoset embryonic stem cell (ESC) line that ubiquitously expresses the tamoxifen-inducible Cre-driver ERT2CreERT2. We validated the pluripotency of the ESC line and also successfully demonstrated the temporal control of the Cre-driver in a tamoxifen-dependent manner in the ESCs. This ESC line, named ActiCre-B1, will be a valuable resource for in vitro investigation of phenotypes related to embryonic lethality by targeted knockout of functionally important genes.

本文言語English
論文番号102164
ジャーナルStem Cell Research
51
DOI
出版ステータスPublished - 2021 3

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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