Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs)

Munenari Itoh, Noriko Umegaki, Zongyou Guo, Liang Liu, Claire A. Higgins, Angela M. Christiano

研究成果: Article

89 引用 (Scopus)

抄録

Recent generation of patient-specific induced pluripotent stem cells (PS-iPSCs) provides significant advantages for cell- and gene-based therapy. Establishment of iPSC-based therapy for skin diseases requires efficient methodology for differentiating iPSCs into both keratinocytes and fibroblasts, the major cellular components of the skin, as well as the reconstruction of skin structures using these iPSC-derived skin components. We previously reported generation of keratinocytes from human iPSCs for use in the treatment of recessive dystrophic epidermolysis bullosa (RDEB) caused by mutations in the COL7A1 gene. Here, we developed a protocol for differentiating iPSCs into dermal fibroblasts, which also produce type VII collagen and therefore also have the potential to treat RDEB. Moreover, we generated in vitro 3D skin equivalents composed exclusively human iPSC-derived keratinocytes and fibroblasts for disease models and regenerative therapies for skin diseases, first demonstrating that iPSCs can provide the basis for modeling a human organ derived entirely from two different types of iPSC-derived cells.

元の言語English
記事番号e77673
ジャーナルPLoS One
8
発行部数10
DOI
出版物ステータスPublished - 2013 10 11
外部発表Yes

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Induced Pluripotent Stem Cells
Stem cells
skin (animal)
Skin
keratinocytes
Fibroblasts
epidermolysis bullosa
Keratinocytes
Epidermolysis Bullosa Dystrophica
fibroblasts
skin diseases
Skin Diseases
therapeutics
Genes
Collagen Type VII
induced pluripotent stem cells
disease models
Cell- and Tissue-Based Therapy
Genetic Therapy
collagen

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Itoh, M., Umegaki, N., Guo, Z., Liu, L., Higgins, C. A., & Christiano, A. M. (2013). Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs). PLoS One, 8(10), [e77673]. https://doi.org/10.1371/journal.pone.0077673

Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs). / Itoh, Munenari; Umegaki, Noriko; Guo, Zongyou; Liu, Liang; Higgins, Claire A.; Christiano, Angela M.

:: PLoS One, 巻 8, 番号 10, e77673, 11.10.2013.

研究成果: Article

Itoh, M, Umegaki, N, Guo, Z, Liu, L, Higgins, CA & Christiano, AM 2013, 'Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs)', PLoS One, 巻. 8, 番号 10, e77673. https://doi.org/10.1371/journal.pone.0077673
Itoh, Munenari ; Umegaki, Noriko ; Guo, Zongyou ; Liu, Liang ; Higgins, Claire A. ; Christiano, Angela M. / Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs). :: PLoS One. 2013 ; 巻 8, 番号 10.
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