Generation of a bioengineered lacrimal gland by using the organ germ method

Masatoshi Hirayama, Kazuo Tsubota, Takashi Tsuji

研究成果: Chapter

3 被引用数 (Scopus)

抄録

In organogenesis including lacrimal gland development, cell arrangement within a tissue plays an important role. The lacrimal gland develops from embryonic ocular surface epithelium through reciprocal epithelial and mesenchymal interaction, which is organized by interactive regulation of various pathways of signaling molecules. Current development of an in vitro three-dimensional cell manipulation procedure to generate a bioengineered organ germ, named as the organ germ method, has shown the regeneration of a histologically correct and fully functional bioengineered lacrimal gland after engraftment in vivo. This method demonstrated a possibility of lacrimal gland organ replacement to treat dry eye disease, which has been a public health problem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ153-165
ページ数13
1597
DOI
出版ステータスPublished - 2017

出版物シリーズ

名前Methods in Molecular Biology
1597
ISSN(印刷版)10643745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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