@article{e608ee9359f946218b636820570a6f49,
title = "Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems",
abstract = "BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.",
author = "Sho Yoshimatsu and Tsukika Sato and Masafumi Yamamoto and Erika Sasaki and Mayutaka Nakajima and Mari Nakamura and Seiji Shiozawa and Toshiaki Noce and Hideyuki Okano",
note = "Funding Information: The authors would like to express their thanks for Kanae Ohtsu, Yuka Hiroi (Keio Univeristy), and Haruka Shinohara (CIEA) for assisting this study. They would also like to thank all the laboratory members of H.O. for their encouragement and generous support. Data presented herein were obtained in the “Construction of System for Spread of Primate Model Animals” project, performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP18dm0207002 to H.O.); and Scientific Research in Innovative Areas, a MEXT Grant-in-Aid project FY2014-2018 “Brain Protein Aging and Dementia Control” (Grant ID: 26117007 to H.O.). This study was also supported by Grant-in-Aid for JSPS Fellows (Grant ID: 19J12871 to S.Y.) and RIKEN Junior Research Associate Program (to S.Y.). Funding Information: The authors would like to express their thanks for Kanae Ohtsu, Yuka Hiroi (Keio Univeristy), and Haruka Shinohara (CIEA) for assisting this study. They would also like to thank all the laboratory members of H.O. for their encouragement and generous support. Data presented herein were obtained in the “ Construction of System for Spread of Primate Model Animals ” project, performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP18dm0207002 to H.O.); and Scientific Research in Innovative Areas, a MEXT Grant-in-Aid project FY2014-2018 “ Brain Protein Aging and Dementia Control ” (Grant ID: 26117007 to H.O.). This study was also supported by Grant-in-Aid for JSPS Fellows (Grant ID: 19J12871 to S.Y.) and RIKEN Junior Research Associate Program (to S.Y.). Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = apr,
doi = "10.1016/j.scr.2020.101740",
language = "English",
volume = "44",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
}