Generation of a monoclonal antibody that induces apoptosis of hematopoietic cells

Hiroyuki Mio, Mitsuo Satoh, Sachiko Yokokawa, Hitoshi Nishio, Toshio Suda, Tatsuya Tamaoki

研究成果: Article査読

抄録

Here, we developed anti-murine myeloid cell (NFS-60 cell) monoclonal antibodies in order to characterize the further mechanism of cell-to-cell interaction between the stromal cells and the hematopoietic progenitor cells. The established antibody, designated mAb UNF, inhibited proliferation of NFS-60 cells and induced apoptosis. Incubation of NFS-60 cells with mAb UNF initiated the cell aggregation within 3 hours. This phenomena did not require newly synthesized proteins, since the pretreatment of 1 mM cycloheximide failed to prevent the agglutination. Fifty-eight percent of mouse hematopoietic stem cells, Lin-, c-Kit+, Sca-1+ bone marrow cells, were shown to express UNF antigen. The glycolipid prepared from NFS-60 cells was specifically immunostained by mAb UNF.

本文言語English
ページ(範囲)727-731
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
243
3
DOI
出版ステータスPublished - 1998 2 24

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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