TY - JOUR
T1 - Generation of highly homogeneous strains of zebrafish through full sib-pair mating
AU - Shinya, Minori
AU - Sakai, Noriyoshi
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011/10
Y1 - 2011/10
N2 - Genetically homogeneous populations, such as inbred strains, are powerful experimental tools that are ideally suited for studying immunology, cancer, and genetics of complex traits. The zebrafish, Danio rerio, has been underutilized in these research areas because homogeneous strains of experimental fish have not been available in tractable condition. Here, we attempted to inbreed two zebrafish wild-type strains, Tübingen and India, through full sib-pair mating. Although the inbred Tübingen strain failed to thrive and was lost after 13 generations, an inbred India strain (IM) has been maintained successfully. The IM strain has endured 16 generations of inbreeding and has maintained a healthy condition. Two additional strains, IM12m and IM14m, were established as closed colonies from the branches of the IM strain. Genotype analyses using genetic markers revealed a dramatic decrease in polymorphisms (62% dropped to 5%) in both IM (generation 14) and the two closed colonies. This indicates a high level of homogeneity in these strains. Furthermore, scale transplantations between individuals within each strain were successful. These data suggest that extremely homogeneous zebrafish strains have been established, thereby creating a valuable resource for practical application.
AB - Genetically homogeneous populations, such as inbred strains, are powerful experimental tools that are ideally suited for studying immunology, cancer, and genetics of complex traits. The zebrafish, Danio rerio, has been underutilized in these research areas because homogeneous strains of experimental fish have not been available in tractable condition. Here, we attempted to inbreed two zebrafish wild-type strains, Tübingen and India, through full sib-pair mating. Although the inbred Tübingen strain failed to thrive and was lost after 13 generations, an inbred India strain (IM) has been maintained successfully. The IM strain has endured 16 generations of inbreeding and has maintained a healthy condition. Two additional strains, IM12m and IM14m, were established as closed colonies from the branches of the IM strain. Genotype analyses using genetic markers revealed a dramatic decrease in polymorphisms (62% dropped to 5%) in both IM (generation 14) and the two closed colonies. This indicates a high level of homogeneity in these strains. Furthermore, scale transplantations between individuals within each strain were successful. These data suggest that extremely homogeneous zebrafish strains have been established, thereby creating a valuable resource for practical application.
KW - Danio rerio
KW - Inbreeding depression
KW - Loss of heterogeneity
KW - Sib-pair mating
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U2 - 10.1534/g3.111.000851
DO - 10.1534/g3.111.000851
M3 - Article
C2 - 22384348
AN - SCOPUS:84858333045
VL - 1
SP - 377
EP - 386
JO - G3: Genes, Genomes, Genetics
JF - G3: Genes, Genomes, Genetics
SN - 2160-1836
IS - 5
ER -