Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells

Satoru Yoshida, Miyuki Yasuda, Hideyuki Miyashita, Yoko Ogawa, Tetsu Yoshida, Yumi Matsuzaki, Kazuo Tsubota, Hideyuki Okano, Shigeto Shimmura

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Background: Application of induced pluripotent stem (iPS) cells in regenerative medicine will bypass ethical issues associated with use of embryonic stem cells. In addition, patient-specific IPS cells can be useful to elucidate the pathophysiology of genetic disorders, drug screening, and tailor-made medicine. However, in order to apply iPS cells to mitotic tissue, induction of tissue stem cells that give rise to progeny of the target organ is required. Methodology/Principal Findings: We induced stratified epithelial cells from mouse iPS cells by co-culture with PA6 feeder cells (SDIA-method) with use of BMP4. Clusters of cells positive for the differentiation markers KRT1 or KRT12 were observed in KRT14-positive colonies. We successfully cloned KRT14 and p63 double-positive stratified epithelial progenitor cells from iPS-derived epithelial cells, which formed stratified epithelial sheets consisting of five- to six-polarized epithelial cells in vitro. When these clonal cells were cultured on denuded mouse corneas, a robust stratified epithelial layer was observed with physiological cell polarity including high levels of E-cadherin, p63 and K15 expression in the basal layer and ZO-1 in the superficial layer, recapitulating the apico-basal polarity of the epithelium in vivo. Conclusions/Significance: These results suggest that KRT14 and p63 double-positive epithelial progenitor cells can be cloned from iPS cells in order to produce polarized multilayer epithelial cell sheets.

本文言語English
論文番号e28856
ジャーナルPloS one
6
12
DOI
出版ステータスPublished - 2011 12 9

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル