Generation of transgenic non-human primates with germline transmission

Erika Sasaki, Hiroshi Suemizu, Akiko Shimada, Kisaburo Hanazawa, Ryo Oiwa, Michiko Kamioka, Ikuo Tomioka, Yusuke Sotomaru, Reiko Hirakawa, Tomoo Eto, Seiji Shiozawa, Takuji Maeda, Mamoru Ito, Ryoji Ito, Chika Kito, Chie Yagihashi, Kenji Kawai, Hiroyuki Miyoshi, Yoshikuni Tanioka, Norikazu TamaokiSonoko Habu, Hideyuki Okano, Tatsuji Nomura

研究成果: Article査読

489 被引用数 (Scopus)

抄録

The common marmoset (Callithrix jacchus) is increasingly attractive for use as a non-human primate animal model in biomedical research. It has a relatively high reproduction rate for a primate, making it potentially suitable for transgenic modification. Although several attempts have been made to produce non-human transgenic primates, transgene expression in the somatic tissues of live infants has not been demonstrated by objective analyses such as polymerase chain reaction with reverse transcription or western blots. Here we show that the injection of a self-inactivating lentiviral vector in sucrose solution into marmoset embryos results in transgenic common marmosets that expressed the transgene in several organs. Notably, we achieved germline transmission of the transgene, and the transgenic offspring developed normally. The successful creation of transgenic marmosets provides a new animal model for human disease that has the great advantage of a close genetic relationship with humans. This model will be valuable to many fields of biomedical research.

本文言語English
ページ(範囲)523-527
ページ数5
ジャーナルNature
459
7246
DOI
出版ステータスPublished - 2009 5 28

ASJC Scopus subject areas

  • 一般

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