Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

Kazuo Sugiyama, Kenji Suzuki, Takahide Nakazawa, Kenji Funami, Takayuki Hishiki, Kazuya Ogawa, Satoru Saito, Kumiko W. Shimotohno, Takeshi Suzuki, Yuko Shimizu, Reiri Tobita, Makoto Hijikata, Hiroshi Takaku, Kunitada Shimotohno

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

本文言語English
ページ(範囲)6922-6928
ページ数7
ジャーナルJournal of Virology
83
13
DOI
出版ステータスPublished - 2009 7月

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • 昆虫科学
  • ウイルス学

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