Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. populations using newly developed microsatellite markers

Yoshikuni Hodoki, Kako Ohbayashi, Hidenobu Kunii

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Nine microsatellite loci were isolated and characterized from the clonal salt-marsh sedge Carex scabrifolia, and genetic diversities within four populations were analyzed. The number of alleles per locus ranged from 2 to 7, with an average of 4.7. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.679, respectively. In two populations, almost all polymorphic loci showed a significant excess of heterozygotes due to their high clonal reproduction and low number of genotypes (genets) composing the populations. Thus, the number of identical genets varied greatly among populations and ranged from 1 to 28, irrespective of population size (i.e., population area and ramet number). When attempting to conserve and restore clonal plants such as C. scabrifolia, it is important to preserve sufficient genetic diversity within a population, which can be assessed using genetic markers such as the simple sequence repeat markers described here.

本文言語English
ページ(範囲)1361-1364
ページ数4
ジャーナルConservation Genetics
10
5
DOI
出版ステータスPublished - 2009 9

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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