Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry: Detection of five base deletion from blood DNA of a familial adenomatous polyposis patient

Yasuhiro Naito, Keiichiro Ishikawa, Yoshinori Koga, Toshihiro Tsuneyoshi, Hideya Terunuma, Ryuichi Arakawa

研究成果: Article査読

25 被引用数 (Scopus)

抄録

A 5-base deleted mutation of adenomatous polyposis coli (APC) gene was detected by using electrospray ionization mass spectrometry of polymerase chain reaction (PCR) products. Genomic DNA was extracted from a familial adenomatous polyposis patient blood, and a 57-base pairs segment of APC gene was amplified by PCR. The PCR products were purified, digested with restriction endonuclease, purified, and determined by electrospray mass spectrometry.

本文言語English
ページ(範囲)737-742
ページ数6
ジャーナルJournal of the American Society for Mass Spectrometry
8
7
DOI
出版ステータスPublished - 1997 7 1
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 分光学

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