Genetic mapping of 40 cDNA clones on the mouse genome by PCR

M. S.H. Ko, X. Wang, J. H. Horton, M. D. Hagen, N. Takahashi, Y. Maezaki, J. H. Nadeau

研究成果: Article査読

28 被引用数 (Scopus)

抄録

We recently proposed a new PCR-based genetic marker assay for the mouse genome that exploits sequence differences in the 3′-untranslated region (UTR) of cDNAs between different mouse strains, called "biallelic polymorphic expressed sequence tags (bESTs)." The specific use of 3′-UTR has several advantages: (1) frequent sequence polymorphism between different mouse strains, (2) most commonly uninterrupted by introns, (3) usually unique sequence even among closely related gene family members. In this paper, we identify additional genetic loci defined by bEST and determine their location on the mouse genetic map by using interspecific backross mapping panels between C57BL/6J and Mus spretus. Of 136 markers tested, 86 produced unique PCR products from C57BL/6J and M. spretus genomic DNAs. We then sequenced these 86 PCR products from C57BL/6J and M. spretus and found that 59 markers have sequence polymorphisms. Of these, we mapped 36 by restriction fragment length polymorphism (RFLP) of the PCR products and 4 by length polymorphism (LP) of the PCR products. We discuss the possibility of a large-scale application of this method for cDNA mapping.

本文言語English
ページ(範囲)349-355
ページ数7
ジャーナルMammalian Genome
5
6
DOI
出版ステータスPublished - 1994 6月
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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