Genome-wide distribution of linker histone H1.0 is independent of MeCP2

Aya Ito-Ishida, Hari Krishna Yamalanchili, Yingyao Shao, Steven A. Baker, Laura D. Heckman, Laura A. Lavery, Ji Yoen Kim, Laura M. Lombardi, Yaling Sun, Zhandong Liu, Huda Y. Zoghbi

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.

本文言語English
ページ(範囲)794-798
ページ数5
ジャーナルNature Neuroscience
21
6
DOI
出版ステータスPublished - 2018 6月 1

ASJC Scopus subject areas

  • 神経科学(全般)

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