TY - JOUR
T1 - Genomic approaches to early embryogenesis and stem cell biology
AU - Aiba, Kazuhiro
AU - Carter, Mark G.
AU - Matoba, Ryo
AU - Ko, Minoru S.H.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/11
Y1 - 2006/11
N2 - Large-scale systematic gene expression analyses of early embryos and stem cells provide useful information to identify genes expressed differentially or uniquely in these cells. We review the current status of various approaches applied to preimplantation embryos and stem cells: expressed sequence tag, serial analysis of gene expression, differential display, massively parallel signature sequencing, DNA microarray (DNA chip) analysis, and chromatin-immunoprecipitation microarrays. We also discuss the biological questions that can only be addressed by the analysis of global gene expression patterns, such as so-called stemness and developmental potency. As the emphasis now shifts from expression profiling to functional studies, we review the genome-scale functional studies of genes: expression cloning, gene trapping, RNA interference, and gene disruptions. Finally, we discuss the future clinical application of such methodologies.
AB - Large-scale systematic gene expression analyses of early embryos and stem cells provide useful information to identify genes expressed differentially or uniquely in these cells. We review the current status of various approaches applied to preimplantation embryos and stem cells: expressed sequence tag, serial analysis of gene expression, differential display, massively parallel signature sequencing, DNA microarray (DNA chip) analysis, and chromatin-immunoprecipitation microarrays. We also discuss the biological questions that can only be addressed by the analysis of global gene expression patterns, such as so-called stemness and developmental potency. As the emphasis now shifts from expression profiling to functional studies, we review the genome-scale functional studies of genes: expression cloning, gene trapping, RNA interference, and gene disruptions. Finally, we discuss the future clinical application of such methodologies.
KW - Embryogenomics
KW - Large-scale analysis
KW - Microarray
KW - Preimplantation embryos
KW - Stem cells
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U2 - 10.1055/s-2006-952155
DO - 10.1055/s-2006-952155
M3 - Review article
C2 - 17123228
AN - SCOPUS:33845306470
VL - 24
SP - 330
EP - 339
JO - Seminars in Reproductive Medicine
JF - Seminars in Reproductive Medicine
SN - 1526-8004
IS - 5
ER -