Genomic evidence for convergent evolution of gene clusters for momilactone biosynthesis in land plants

Lingfeng Mao, Hiroshi Kawaide, Toshiya Higuchi, Meihong Chen, Koji Miyamoto, Yoshiki Hirata, Honoka Kimura, Sho Miyazaki, Miyu Teruya, Kaoru Fujiwara, Keisuke Tomita, Hisakazu Yamane, Ken Ichiro Hayashi, Hideaki Nojiri, Lei Jia, Jie Qiu, Chuyu Ye, Michael P. Timko, Longjiang Fan, Kazunori Okada

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Momilactones are bioactive diterpenoids that contribute to plant defense against pathogens and allelopathic interactions between plants. Both cultivated and wild grass species of Oryza and Echinochloa crus-galli (barnyard grass) produce momilactones using a biosynthetic gene cluster (BGC) in their genomes. The bryophyte Calohypnum plumiforme (formerly Hypnum plumaeforme) also produces momilactones, and the bifunctional diterpene cyclase gene CpDTC1/HpDTC1, which is responsible for the production of the diterpene framework, has been characterized. To understand the molecular architecture of the momilactone biosynthetic genes in the moss genome and their evolutionary relationships with other momilactone-producing plants, we sequenced and annotated the C. plumiforme genome. The data revealed a 150-kb genomic region that contains two cytochrome P450 genes, the CpDTC1/HpDTC1 gene and the "dehydrogenase momilactone A synthase" gene tandemly arranged and inductively transcribed following stress exposure. The predicted enzymatic functions in yeast and recombinant assay and the successful pathway reconstitution in Nicotiana benthamiana suggest that it is a functional BGC responsible for momilactone production. Furthermore, in a survey of genomic sequences of a broad range of plant species, we found that momilactone BGC is limited to the two grasses (Oryza and Echinochloa) and C. plumiforme, with no synteny among these genomes. These results indicate that while the gene cluster in C. plumiforme is functionally similar to that in rice and barnyard grass, it is likely a product of convergent evolution. To the best of our knowledge, this report of a BGC for a specialized plant defense metabolite in bryophytes is unique.

本文言語English
ページ(範囲)12472-12480
ページ数9
ジャーナルProceedings of the National Academy of Sciences of the United States of America
117
22
DOI
出版ステータスPublished - 2020 6 2
外部発表はい

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Genomic evidence for convergent evolution of gene clusters for momilactone biosynthesis in land plants」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル