Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries

N. Doi, H. Yanagawa

研究成果: Review article

11 被引用数 (Scopus)

抄録

The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establishment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types; that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.

本文言語English
ページ(範囲)497-509
ページ数13
ジャーナルCombinatorial Chemistry and High Throughput Screening
4
6
DOI
出版ステータスPublished - 2001 1 1

ASJC Scopus subject areas

  • Drug Discovery
  • Computer Science Applications
  • Organic Chemistry

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