Giardia lamblia Transcriptome Analysis Using TSS-Seq and RNA-Seq

Mohammed E.M. Tolba, Seiki Kobayashi, Mihoko Imada, Yutaka Suzuki, Sumio Sugano

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Giardia lamblia is a protozoan parasite that is found worldwide and has both medical and veterinary importance. We applied the transcription start sequence (TSS-seq) and RNA sequence (RNA-seq) techniques to study the transcriptome of the assemblage A WB strain trophozoite. We identified 8000 transcription regions (TR) with significant transcription. Of these regions, 1881 TRs were more than 500 nucleotides upstream of an annotated ORF. Combining both techniques helped us to identify 24 ORFs that should be re-annotated and 60 new ORFs. From the 8000 TRs, we were able to identify an AT-rich consensus that includes the transcription initiation site. It is possible that transcription that was previously thought to be bidirectional is actually unidirectional.

本文言語English
論文番号e76184
ジャーナルPloS one
8
10
DOI
出版ステータスPublished - 2013 10 7

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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