Glial cell degeneration and hypomyelination caused by overexpression of myelin proteolipid protein gene

Tetsushi Kagawa, Kazuhiro Ikenaka, Yoshiro Inoue, Shigeki Kuriyama, Tadasu Tsujii, Junji Nakao, Kazunori Nakajima, Jun Aruga, Hideyuki Okano, Katsuhiko Mikoshiba

研究成果: Article

224 引用 (Scopus)

抜粋

Myelin proteolipid protein (PLP), the major myelin protein in the CNS, has been thought to function in myelin assembly. Thus, mutations within the gene coding for PLP (Plp) cause hypomyelination, such as the jimpy phenotype in mice and Pelizaeus-Merzbacher disease in humans. However, these mutants often exhibit premature death of oligodendrocytes, which form CNS myelin. To elucidate the functional roles of Pip gene products in the maturation and/or survival of oligodendrocytes, we produced transgenic mice overexpressing the Plp gene by introducing extra wild-type mouse Pip genes. Surprisingly, transgenic mice bearing 4 more Plp genes exhibited dysmyelination in the CNS, whereas those with 2 more Plp genes showed normal myelination at an early age (3 weeks after birth), but later developed demyelination. Overexpression of the Plp gene resulted in arrested maturation of oligodendrocytes, and the severity of arrest was dependent on the extent of overexpression. Overexpression also led to oligodendrocyte cell death, apparently caused by abnormal swelling of the Golgi apparatus. Thus, tight regulation of Plpgene expression is necessary for normal oligodendrocyte differentiation and survival, and its overexpression can be the cause of both dys- and demyelination.

元の言語English
ページ(範囲)427-442
ページ数16
ジャーナルNeuron
13
発行部数2
DOI
出版物ステータスPublished - 1994 8
外部発表Yes

ASJC Scopus subject areas

  • Neuroscience(all)

フィンガープリント Glial cell degeneration and hypomyelination caused by overexpression of myelin proteolipid protein gene' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Kagawa, T., Ikenaka, K., Inoue, Y., Kuriyama, S., Tsujii, T., Nakao, J., Nakajima, K., Aruga, J., Okano, H., & Mikoshiba, K. (1994). Glial cell degeneration and hypomyelination caused by overexpression of myelin proteolipid protein gene. Neuron, 13(2), 427-442. https://doi.org/10.1016/0896-6273(94)90358-1