Global metabolic network reorganization by adaptive mutations allows fast growth of Escherichia coli on glycerol

Kian Kai Cheng, Baek Seok Lee, Takeshi Masuda, Takuro Ito, Kazutaka Ikeda, Akiyoshi Hirayama, Lingli Deng, Jiyang Dong, Kazuyuki Shimizu, Tomoyoshi Soga, Masaru Tomita, Bernhard O. Palsson, Martin Robert

研究成果: Article査読

59 被引用数 (Scopus)

抄録

Comparative whole-genome sequencing enables the identification of specific mutations during adaptation of bacteria to new environments and allelic replacement can establish their causality. However, the mechanisms of action are hard to decipher and little has been achieved for epistatic mutations, especially at the metabolic level. Here we show that a strain of Escherichia coli carrying mutations in the rpoC and glpK genes, derived from adaptation in glycerol, uses two distinct metabolic strategies to gain growth advantage. A 27-bp deletion in the rpoC gene first increases metabolic efficiency. Then, a point mutation in the glpK gene promotes growth by improving glycerol utilization but results in increased carbon wasting as overflow metabolism. In a strain carrying both mutations, these contrasting carbon/energy saving and wasting mechanisms work together to give an 89% increase in growth rate. This study provides insight into metabolic reprogramming during adaptive laboratory evolution for fast cellular growth.

本文言語English
論文番号3233
ジャーナルNature communications
5
DOI
出版ステータスPublished - 2014 1月 31

ASJC Scopus subject areas

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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