@article{4d70385a10de4371920af2e5a6461350,
title = "Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses",
abstract = "We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 program provides information on known and unknown complex lipid molecules. The FBMN technique clusters similar MS2 spectra, facilitating the identification of bacterial lipids. Targeted analysis was used as a complementary method to cover oxylipins. Here, we provide details for targeted and untargeted analyses. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2020).",
keywords = "Immunology, Mass Spectrometry, Metabolomics, Microbiology",
author = "Nobuyuki Okahashi and Masahiro Ueda and Shu Yasuda and Hiroshi Tsugawa and Makoto Arita",
note = "Funding Information: This work was supported by the AMED LEAP (JP18gm0010003 to M.A.) and JSPS Grant-in-Aid for Scientific Research on Innovative Areas {\textquoteleft}{\textquoteleft}LipoQuality{\textquoteright}{\textquoteright} (15H05897 and 15H05898 to M.A.) and JSPS KAKENHI (18H02432 and 18K19155 to H.T.). We thank our lab members, especially Kazutaka Ikeda for the development of untargeted lipidomics system, and Mie Honda, Aya Hori, Kanako Igarashi, and Mimi Mitsuji for their skillful technical support. N.O. organized the protocol design. M.U. wrote the sample preparation protocol. S.Y. wrote the mouse experiment protocol. N.O. M.U. and S.Y. wrote the LC-MS/MS method protocols for targeted and untargeted lipidomics. H.T. wrote the data processing and molecular networking protocols. M.A. initiated and designed this work, and all authors wrote the manuscript. The authors declare no competing interests. Funding Information: This work was supported by the AMED LEAP ( JP18gm0010003 to M.A.) and JSPS Grant-in-Aid for Scientific Research on Innovative Areas {\textquoteleft}{\textquoteleft}LipoQuality{\textquoteright}{\textquoteright} ( 15H05897 and 15H05898 to M.A.) and JSPS KAKENHI ( 18H02432 and 18K19155 to H.T.). We thank our lab members, especially Kazutaka Ikeda for the development of untargeted lipidomics system, and Mie Honda, Aya Hori, Kanako Igarashi, and Mimi Mitsuji for their skillful technical support. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100492",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}