Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses

Nobuyuki Okahashi, Masahiro Ueda, Shu Yasuda, Hiroshi Tsugawa, Makoto Arita

研究成果: Article査読

抄録

We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 program provides information on known and unknown complex lipid molecules. The FBMN technique clusters similar MS2 spectra, facilitating the identification of bacterial lipids. Targeted analysis was used as a complementary method to cover oxylipins. Here, we provide details for targeted and untargeted analyses. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2020).

本文言語English
論文番号100492
ジャーナルSTAR Protocols
2
2
DOI
出版ステータスPublished - 2021 6 18

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 神経科学(全般)
  • 免疫学および微生物学(全般)

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