Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis

Yuji Tanaka, Takahisa Ogawa, Takanori Maruta, Yuta Yoshida, Kazuharu Arakawa, Takahiro Ishikawa

研究成果: Article査読

27 被引用数 (Scopus)

抄録

The phytoflagellate Euglena gracilis produces a large amount of paramylon (PM), a conglomerate of liner β-1,3-glucan chains, as a storage polysaccharide. PM is synthesized from uridine diphosphate-glucose, but its mechanism of formation is largely unknown. Two enzymes, glucan synthase-like (EgGSL) 1 and EgGSL2 were previously identified as candidates for PM synthesis in a Euglena transcriptome analysis. Here, we performed a reverse genetic analysis on these enzymes. Knockdown of EgGSL2, but not EgGSL1, significantly inhibits PM accumulation in Euglena cells. Additionally, β-1,3-glucan synthesis is detected in a PM-associated membrane fraction extracted from Euglena cells. Our findings indicate that EgGSL2 is the predominant enzyme for PM biosynthesis.

本文言語English
ページ(範囲)1360-1370
ページ数11
ジャーナルFEBS Letters
591
10
DOI
出版ステータスPublished - 2017 5 1

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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