Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach

Zheng Ming Wang, Roman Aizman, Lena Grahnquist, Masato Yasui, Åsa Hemphälä, Gianni Celsi

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The development of gastric H,K-ATPase from fetal to adult life was studied in the rat. The α and β H,K-ATPase mRNA abundance, the protein abundance, and the enzyme activity increased postnatally. The sharpest increase in mRNA and enzyme activity was observed in the weaning period. Several intestinal enzymes are known to be stimulated by glucocorticoids at the time of weaning. To study the role of glucocorticoids in the maturation of gastric H,K-ATPase, we treated 10-d-old rats with a single injection of betamethasone. Twenty-four hours after betamethasone injection, the enzyme activity was significantly higher than in the control animals (2.6-fold, p < 0.05). The abundance of catalytic α H,K-ATPase protein was also increased (2.5-fold, p < 0.01). The time-dependent effect of betamethasone on α H,K- ATPase mRNA was determined from 6 to 24 h after treatment. Glucocorticoids did not significantly alter the mRNA abundance within 18 h. Twenty four hours after injection, the gastric H,K-ATPase mRNA was significantly increased compared with controls (2.8- and 2.2-fold increase for α and β subunits, respectively, p < 0.01 for both). In conclusion this study indicates that glucocorticoids may regulate the long term maturation of gastric H,K-ATPase by indirectly stimulating enzyme synthesis.

本文言語English
ページ(範囲)658-663
ページ数6
ジャーナルPediatric Research
40
5
DOI
出版ステータスPublished - 1996 11月
外部発表はい

ASJC Scopus subject areas

  • 小児科学、周産期医学および子どもの健康

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