Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis

Midori Matsumoto, Shun ichi Kurata, Hirokazu Fujimoto, Motonori Hoshi

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Protamine 1 and heat shock cognate 70 kDa protein (hsc70t) are known to be synthesized in haploid cells during spermatogenesis, and the mRNAs of these proteins have also been shown to accumulate in the haploid cells. However, it is unknown at which stage of spermatogenesis the genes for these proteins are actually activated. To examine this problem, we fractionated mouse adult testes cells at four different developmental stages, extracted their nuclei and carried out run-off assays with hsc70t and protamine 1 DNA probes. Results showed that both genes are mainly activated at the round spermatid stage. As the protein products of these genes accumulate at the later stage, it is interesting that these genes are regulated at the transcriptional and translational levels during spermatogenesis.

本文言語English
ページ(範囲)274-278
ページ数5
ジャーナルBBA - Gene Structure and Expression
1174
3
DOI
出版ステータスPublished - 1993 9月 23
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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