TY - JOUR
T1 - Heart regeneration using reprogramming technology
AU - Ieda, Masaki
PY - 2013
Y1 - 2013
N2 - Loss of terminally differentiated cardiomyocytes due to heart disease is irreversible and current therapeutic regimes are limited. Cell therapy using stem cell-derived cardiomyocytes is an attractive option to repair injured hearts. The discovery of direct reprogramming of broblasts into induced pluripotent stem cells (iPSCs) and successful differentiation of iPSCs into cardiomyocytes provided a revolutionary paradigm in heart regenerative research. During the past decades, signicant advances in stem cell culture, differentiation and purication protocols, as well as in cell transplantation methodologies, have been achieved. On the other hand, recent studies demonstrated that a somatic cell could be converted into an alternative differentiated cell type without rst becoming a stem cell by overexpression of lineage-specic factors. We found that functional cardiomyocytes can be directly induced from broblasts by a combination of three cardiac transcription factors, Gata4, Mef2c and Tbx5, in vitro and in vivo. I will review the perspectives of heart regeneration using reprogramming technology.
AB - Loss of terminally differentiated cardiomyocytes due to heart disease is irreversible and current therapeutic regimes are limited. Cell therapy using stem cell-derived cardiomyocytes is an attractive option to repair injured hearts. The discovery of direct reprogramming of broblasts into induced pluripotent stem cells (iPSCs) and successful differentiation of iPSCs into cardiomyocytes provided a revolutionary paradigm in heart regenerative research. During the past decades, signicant advances in stem cell culture, differentiation and purication protocols, as well as in cell transplantation methodologies, have been achieved. On the other hand, recent studies demonstrated that a somatic cell could be converted into an alternative differentiated cell type without rst becoming a stem cell by overexpression of lineage-specic factors. We found that functional cardiomyocytes can be directly induced from broblasts by a combination of three cardiac transcription factors, Gata4, Mef2c and Tbx5, in vitro and in vivo. I will review the perspectives of heart regeneration using reprogramming technology.
KW - Heart
KW - Heart regeneration
KW - IPS cell
KW - Induced cardiomyocyte
KW - Reprogramming
UR - http://www.scopus.com/inward/record.url?scp=84875773584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875773584&partnerID=8YFLogxK
U2 - 10.2183/pjab.89.118
DO - 10.2183/pjab.89.118
M3 - Review article
C2 - 23474887
AN - SCOPUS:84875773584
VL - 89
SP - 118
EP - 128
JO - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
JF - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
SN - 0386-2208
IS - 3
ER -