Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

Shinya Ohata; Makiko Nawa; Takeshi Kasama; Tokiwa Yamasaki; Kenji Sawanobori; Shoji Hata; Takashi Nakamura; Yoichi Asaoka; Toshio Watanabe; Hitoshi Okamoto; Takahiko Hara; Shuji Terai; Isao Sakaida; Toshiaki Katada; Hiroshi Nishina

doi:10.1016/j.bbrc.2008.12.149

Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

Shinya Ohata, Makiko Nawa, Takeshi Kasama, Tokiwa Yamasaki, Kenji Sawanobori, Shoji Hata, Takashi Nakamura, Yoichi Asaoka, Toshio Watanabe, Hitoshi Okamoto, Takahiko Hara, Shuji Terai, Isao Sakaida, Toshiaki Katada, Hiroshi Nishina

研究成果: Article › 査読

16 被引用数 (Scopus)

抄録

The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1^-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

本文言語	English
ページ（範囲）	817-823
ページ数	7
ジャーナル	Biochemical and Biophysical Research Communications
巻	379
号	4
DOI	https://doi.org/10.1016/j.bbrc.2008.12.149
出版ステータス	Published - 2009 2月 20
外部発表	はい

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

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10.1016/j.bbrc.2008.12.149

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引用スタイル

Ohata, S., Nawa, M., Kasama, T., Yamasaki, T., Sawanobori, K., Hata, S., Nakamura, T., Asaoka, Y., Watanabe, T., Okamoto, H., Hara, T., Terai, S., Sakaida, I., Katada, T., & Nishina, H. (2009). Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells. Biochemical and Biophysical Research Communications, 379(4), 817-823. https://doi.org/10.1016/j.bbrc.2008.12.149

Ohata, S, Nawa, M, Kasama, T, Yamasaki, T, Sawanobori, K, Hata, S, Nakamura, T, Asaoka, Y, Watanabe, T, Okamoto, H, Hara, T, Terai, S, Sakaida, I, Katada, T & Nishina, H 2009, 'Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells', Biochemical and Biophysical Research Communications, vol. 379, no. 4, pp. 817-823. https://doi.org/10.1016/j.bbrc.2008.12.149

@article{304fe83520464e85a9df19b6ffaac6e5,

title = "Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells",

abstract = "The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.",

keywords = "AGM, AML1, CD44, Erythrocyte, Hematopoiesis, Hepatoblast, Hepatogenesis, Liv8, Liver, Liver development, Monoclonal antibody",

author = "Shinya Ohata and Makiko Nawa and Takeshi Kasama and Tokiwa Yamasaki and Kenji Sawanobori and Shoji Hata and Takashi Nakamura and Yoichi Asaoka and Toshio Watanabe and Hitoshi Okamoto and Takahiko Hara and Shuji Terai and Isao Sakaida and Toshiaki Katada and Hiroshi Nishina",

note = "Funding Information: This work was supported in part by research grants (to H.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and the Japan Society for the Promotion of Science (JSPS). We are grateful to Drs. Minoru Tanaka, Atsushi Miyajima, Yuki Nakayama, Takahiro Ito, and numerous members of the Nishina and Katada laboratories for critical reading and helpful discussions. ",

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AU - Ohata, Shinya

AU - Nawa, Makiko

AU - Kasama, Takeshi

AU - Yamasaki, Tokiwa

AU - Sawanobori, Kenji

AU - Hata, Shoji

AU - Nakamura, Takashi

AU - Asaoka, Yoichi

AU - Watanabe, Toshio

AU - Okamoto, Hitoshi

AU - Hara, Takahiko

AU - Terai, Shuji

AU - Sakaida, Isao

AU - Katada, Toshiaki

AU - Nishina, Hiroshi

N1 - Funding Information: This work was supported in part by research grants (to H.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and the Japan Society for the Promotion of Science (JSPS). We are grateful to Drs. Minoru Tanaka, Atsushi Miyajima, Yuki Nakayama, Takahiro Ito, and numerous members of the Nishina and Katada laboratories for critical reading and helpful discussions.

PY - 2009/2/20

Y1 - 2009/2/20

N2 - The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

AB - The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

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KW - AML1

KW - CD44

KW - Erythrocyte

KW - Hematopoiesis

KW - Hepatoblast

KW - Hepatogenesis

KW - Liv8

KW - Liver

KW - Liver development

KW - Monoclonal antibody

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

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