Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells

Yohko Kawai, Yutaka Matsumoto, Kiyoaki Watanabe, Hiroshi Yamamoto, Kumi Satoh, Mitsuru Murata, Makoto Handa, Yasuo Ikeda

研究成果: Article

66 引用 (Scopus)

抄録

We investigated the effects of hemodynamic force on fibrinolytic activity of cultured human umbilical vein endothelial cells stimulated by cytokines, using a modified cone-plate viscometer in which well-controlled and -defined sheer forces were generated. Treatment of the cells with interleukin (IL)-β or tumor necrosis factor α (TNFα) under static conditions had no effect on tissue plasminogen activator (t-PA) secretion, while release of plasminogen activator inhibitor-1 (PAI-1) increased. When cells were exposed to increasing shear stress up to 24 dynes/cm2, levels of t-PA and t-PA/PAI-1 complex significantly increased relative to shear stress, while total PAI-1 and active PAI-1 secretion decreased gradually. In the presence of IL-1β or TNFα, the increase in production of t-PA and the t-PA/PAI-1 complex was further augmented. Dot blot hybridization analysis of cultured cells in similar experimental conditions using t-PA and PAI-1 cDNA probes revealed no t-PA mRNA in 3 μg total RNA from static endothelial cells under resting or cytokine-stimulated conditions, but abundant t-PA mRNA was detected in cells subjected to a shear force of 18 dynes/cm2, and the increase was further augmented by addition of cytokines. In contrast, PAI-1 mRNA was detected in resting and cytokine-stimulated, nonsheared endothelial cells, but levels decreased after exposure to shear stress, even in the presence of cytokines. These results indicate a role for hemodynamic forces in regulating fibrinolytic activity with or without cytokine stimulation.

元の言語English
ページ(範囲)2314-2321
ページ数8
ジャーナルBlood
87
発行部数6
出版物ステータスPublished - 1996 3 15

Fingerprint

Endothelial cells
Hemodynamics
Tissue Plasminogen Activator
Plasminogen Activator Inhibitor 1
Endothelial Cells
Cytokines
Shear stress
Messenger RNA
Tumor Necrosis Factor-alpha
Cells
Viscometers
Interleukins
Human Umbilical Vein Endothelial Cells
Interleukin-1
Cones
Cultured Cells
Complementary DNA
RNA

ASJC Scopus subject areas

  • Hematology

これを引用

Kawai, Y., Matsumoto, Y., Watanabe, K., Yamamoto, H., Satoh, K., Murata, M., ... Ikeda, Y. (1996). Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells. Blood, 87(6), 2314-2321.

Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells. / Kawai, Yohko; Matsumoto, Yutaka; Watanabe, Kiyoaki; Yamamoto, Hiroshi; Satoh, Kumi; Murata, Mitsuru; Handa, Makoto; Ikeda, Yasuo.

:: Blood, 巻 87, 番号 6, 15.03.1996, p. 2314-2321.

研究成果: Article

Kawai, Y, Matsumoto, Y, Watanabe, K, Yamamoto, H, Satoh, K, Murata, M, Handa, M & Ikeda, Y 1996, 'Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells', Blood, 巻. 87, 番号 6, pp. 2314-2321.
Kawai Y, Matsumoto Y, Watanabe K, Yamamoto H, Satoh K, Murata M その他. Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells. Blood. 1996 3 15;87(6):2314-2321.
Kawai, Yohko ; Matsumoto, Yutaka ; Watanabe, Kiyoaki ; Yamamoto, Hiroshi ; Satoh, Kumi ; Murata, Mitsuru ; Handa, Makoto ; Ikeda, Yasuo. / Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells. :: Blood. 1996 ; 巻 87, 番号 6. pp. 2314-2321.
@article{f072153e0295447cb1b585be72ec7660,
title = "Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells",
abstract = "We investigated the effects of hemodynamic force on fibrinolytic activity of cultured human umbilical vein endothelial cells stimulated by cytokines, using a modified cone-plate viscometer in which well-controlled and -defined sheer forces were generated. Treatment of the cells with interleukin (IL)-β or tumor necrosis factor α (TNFα) under static conditions had no effect on tissue plasminogen activator (t-PA) secretion, while release of plasminogen activator inhibitor-1 (PAI-1) increased. When cells were exposed to increasing shear stress up to 24 dynes/cm2, levels of t-PA and t-PA/PAI-1 complex significantly increased relative to shear stress, while total PAI-1 and active PAI-1 secretion decreased gradually. In the presence of IL-1β or TNFα, the increase in production of t-PA and the t-PA/PAI-1 complex was further augmented. Dot blot hybridization analysis of cultured cells in similar experimental conditions using t-PA and PAI-1 cDNA probes revealed no t-PA mRNA in 3 μg total RNA from static endothelial cells under resting or cytokine-stimulated conditions, but abundant t-PA mRNA was detected in cells subjected to a shear force of 18 dynes/cm2, and the increase was further augmented by addition of cytokines. In contrast, PAI-1 mRNA was detected in resting and cytokine-stimulated, nonsheared endothelial cells, but levels decreased after exposure to shear stress, even in the presence of cytokines. These results indicate a role for hemodynamic forces in regulating fibrinolytic activity with or without cytokine stimulation.",
author = "Yohko Kawai and Yutaka Matsumoto and Kiyoaki Watanabe and Hiroshi Yamamoto and Kumi Satoh and Mitsuru Murata and Makoto Handa and Yasuo Ikeda",
year = "1996",
month = "3",
day = "15",
language = "English",
volume = "87",
pages = "2314--2321",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "6",

}

TY - JOUR

T1 - Hemodynamic forces modulate the effects of cytokines on fibrinolytic activity of endothelial cells

AU - Kawai, Yohko

AU - Matsumoto, Yutaka

AU - Watanabe, Kiyoaki

AU - Yamamoto, Hiroshi

AU - Satoh, Kumi

AU - Murata, Mitsuru

AU - Handa, Makoto

AU - Ikeda, Yasuo

PY - 1996/3/15

Y1 - 1996/3/15

N2 - We investigated the effects of hemodynamic force on fibrinolytic activity of cultured human umbilical vein endothelial cells stimulated by cytokines, using a modified cone-plate viscometer in which well-controlled and -defined sheer forces were generated. Treatment of the cells with interleukin (IL)-β or tumor necrosis factor α (TNFα) under static conditions had no effect on tissue plasminogen activator (t-PA) secretion, while release of plasminogen activator inhibitor-1 (PAI-1) increased. When cells were exposed to increasing shear stress up to 24 dynes/cm2, levels of t-PA and t-PA/PAI-1 complex significantly increased relative to shear stress, while total PAI-1 and active PAI-1 secretion decreased gradually. In the presence of IL-1β or TNFα, the increase in production of t-PA and the t-PA/PAI-1 complex was further augmented. Dot blot hybridization analysis of cultured cells in similar experimental conditions using t-PA and PAI-1 cDNA probes revealed no t-PA mRNA in 3 μg total RNA from static endothelial cells under resting or cytokine-stimulated conditions, but abundant t-PA mRNA was detected in cells subjected to a shear force of 18 dynes/cm2, and the increase was further augmented by addition of cytokines. In contrast, PAI-1 mRNA was detected in resting and cytokine-stimulated, nonsheared endothelial cells, but levels decreased after exposure to shear stress, even in the presence of cytokines. These results indicate a role for hemodynamic forces in regulating fibrinolytic activity with or without cytokine stimulation.

AB - We investigated the effects of hemodynamic force on fibrinolytic activity of cultured human umbilical vein endothelial cells stimulated by cytokines, using a modified cone-plate viscometer in which well-controlled and -defined sheer forces were generated. Treatment of the cells with interleukin (IL)-β or tumor necrosis factor α (TNFα) under static conditions had no effect on tissue plasminogen activator (t-PA) secretion, while release of plasminogen activator inhibitor-1 (PAI-1) increased. When cells were exposed to increasing shear stress up to 24 dynes/cm2, levels of t-PA and t-PA/PAI-1 complex significantly increased relative to shear stress, while total PAI-1 and active PAI-1 secretion decreased gradually. In the presence of IL-1β or TNFα, the increase in production of t-PA and the t-PA/PAI-1 complex was further augmented. Dot blot hybridization analysis of cultured cells in similar experimental conditions using t-PA and PAI-1 cDNA probes revealed no t-PA mRNA in 3 μg total RNA from static endothelial cells under resting or cytokine-stimulated conditions, but abundant t-PA mRNA was detected in cells subjected to a shear force of 18 dynes/cm2, and the increase was further augmented by addition of cytokines. In contrast, PAI-1 mRNA was detected in resting and cytokine-stimulated, nonsheared endothelial cells, but levels decreased after exposure to shear stress, even in the presence of cytokines. These results indicate a role for hemodynamic forces in regulating fibrinolytic activity with or without cytokine stimulation.

UR - http://www.scopus.com/inward/record.url?scp=0030008270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030008270&partnerID=8YFLogxK

M3 - Article

C2 - 8630393

AN - SCOPUS:0030008270

VL - 87

SP - 2314

EP - 2321

JO - Blood

JF - Blood

SN - 0006-4971

IS - 6

ER -