Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo

Yosuke Okamura, Shinji Takeoka, Yuji Teramura, Hitomi Maruyama, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

研究成果: Article

34 引用 (Scopus)

抄録

BACKGROUND: Prototypes of platelet (PLT) substitutes have been studied and the focus was on a dodecapeptide, HHLGGAKQAGDV (H12), which is a fibrinogen γ-chain carboxy-terminal sequence (γ 400-411) and exists only in the fibrinogen domain. STUDY DESIGN AND METHODS: H12 was conjugated to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles with a mean diameter of 260 ± 60 nm, and the hemostatic ability of H12-conjugated polyAlb (H12-polyAlb) under flow conditions and thrombocytopenic rats have been studied. RESULTS: H12-polyAlb enhanced the in vitro thrombus formation of activated PLTs on a collagen-immobilized plate when exposed to the flowing thrombocytopenic imitation blood. Furthermore, the analysis of the tail bleeding time of rats that were made thrombocytopenic by busulfan injection showed that H12-polyAlb had a hemostatic effect. Based on the bleeding time and the amount injected, the hemostatic capacity of 20 H12-polyAlb was estimated to correspond to that of one PLT. CONCLUSION: These results were important first steps toward the development of PLT substitutes and indicated that H12-polyAlb may be a suitable candidate for an alternative to human PLT concentrates transfused into thrombocytopenic patients in the future.

元の言語English
ページ(範囲)1221-1228
ページ数8
ジャーナルTransfusion
45
発行部数7
DOI
出版物ステータスPublished - 2005 7
外部発表Yes

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Hemostatics
Fibrinogen
Albumins
Blood Platelets
Bleeding Time
Busulfan
Tail
Thrombosis
Collagen
Injections
In Vitro Techniques

ASJC Scopus subject areas

  • Hematology
  • Immunology

これを引用

Okamura, Y., Takeoka, S., Teramura, Y., Maruyama, H., Tsuchida, E., Handa, M., & Ikeda, Y. (2005). Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo. Transfusion, 45(7), 1221-1228. https://doi.org/10.1111/j.1537-2995.2005.00173.x

Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo. / Okamura, Yosuke; Takeoka, Shinji; Teramura, Yuji; Maruyama, Hitomi; Tsuchida, Eishun; Handa, Makoto; Ikeda, Yasuo.

:: Transfusion, 巻 45, 番号 7, 07.2005, p. 1221-1228.

研究成果: Article

Okamura, Y, Takeoka, S, Teramura, Y, Maruyama, H, Tsuchida, E, Handa, M & Ikeda, Y 2005, 'Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo', Transfusion, 巻. 45, 番号 7, pp. 1221-1228. https://doi.org/10.1111/j.1537-2995.2005.00173.x
Okamura, Yosuke ; Takeoka, Shinji ; Teramura, Yuji ; Maruyama, Hitomi ; Tsuchida, Eishun ; Handa, Makoto ; Ikeda, Yasuo. / Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo. :: Transfusion. 2005 ; 巻 45, 番号 7. pp. 1221-1228.
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AU - Tsuchida, Eishun

AU - Handa, Makoto

AU - Ikeda, Yasuo

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