The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||Published - 2003 6 20|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology