Heterogeneity of rat Liver SulfotransferaseS

Michio Matsui, Fusako Nagai

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Sulfotransferases (STs)active on androsterone (AD), cortisol (CS) and 4-nitrophenol (NP) were separated by diethylaminoethyl-cellulose chromatography from cytosolic fractions of female rat liver and were divided into five ST fractions (peaks I-V) with different activities toward three substrates. The precipitates obtained in the 68% of saturation of ammonium sulfate were passed through a Sephadex G-100 column and purified by agarose-hexane adenosine 3\5'-bisphosphate affinity chromatography. AD-ST isoenzyme (peak I) was purified 85-fold, had low CS-ST activity, was devoid of NP-ST activity and appeared to correspond to hydroxysteroid ST 1. Peaks II and V appeared to consist mainly of hydroxysteroid ST and aryl ST, respectively.

本文言語English
ページ(範囲)1048-1053
ページ数6
ジャーナルJournal of Pharmacobio-Dynamics
8
12
DOI
出版ステータスPublished - 1985

ASJC Scopus subject areas

  • 薬理学

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