HHV8-negative primary effusion lymphoma of the peritoneal cavity presenting with a distinct immunohistochemical phenotype

Shinya Tanaka, Harutaka Katano, Kenichi Tsukamoto, Mooran Jin, Sumie Oikawa, Hiroshi Nishihara, Hirofumi Sawa, Kenichi Sawada, Michio Shimizu, Tetsutaro Sata, Yasunori Fujioka, Kazuo Nagashima

研究成果: Article

25 引用 (Scopus)

抄録

Primary effusion lymphoma (PEL) has been recognized as a body-cavity-based lymphoma that was originally reported to be associated with human herpes virus 8 (HHV8) infection, and was frequently found in human immunodeficiency virus-positive (HIV) patients. Here we describe an autopsy case of PEL of the peritoneal cavity in an immunocompetent patient. Cytological analysis of tumor cells within ascites revealed immunocytochemical features of keratin positivity and CD45 negativity. At autopsy, the presence of a massive volume of ascites as well as diffuse tumor cell infiltrates within the serosa of the intestine and mesenterium were observed. Tumor cells were morphologically similar to anaplastic large-cell lymphoma, but were immunohistochemically positive for keratin and epithelial membrane antigen (EMA). They also showed no reactivity to representative lymphocyte surface markers including CD45, in addition to being negative for CD30 and p80NPM/ALK. Molecular analysis of the tumor cells revealed monoclonality of the immunoglobulin heavy-chain gene rearrangement which demonstrated a lymphoma of the B-cell lineage. Furthermore, HHV8 was not detected by immunohistochemical analysis, PCR or nested PCR technique. Based on these results, we consider the present case to be an HHV8-negative PEL with keratin and EMA positivity.

元の言語English
ページ(範囲)293-300
ページ数8
ジャーナルPathology international
51
発行部数4
DOI
出版物ステータスPublished - 2001 6 5
外部発表Yes

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Primary Effusion Lymphoma
Peritoneal Cavity
Keratins
Viruses
Phenotype
Mucin-1
Ascites
Autopsy
Lymphoma
Neoplasms
Immunoglobulin Heavy Chain Genes
Anaplastic Large-Cell Lymphoma
Serous Membrane
Polymerase Chain Reaction
Gene Rearrangement
Cell Lineage
Virus Diseases
Intestines
HIV
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

これを引用

HHV8-negative primary effusion lymphoma of the peritoneal cavity presenting with a distinct immunohistochemical phenotype. / Tanaka, Shinya; Katano, Harutaka; Tsukamoto, Kenichi; Jin, Mooran; Oikawa, Sumie; Nishihara, Hiroshi; Sawa, Hirofumi; Sawada, Kenichi; Shimizu, Michio; Sata, Tetsutaro; Fujioka, Yasunori; Nagashima, Kazuo.

:: Pathology international, 巻 51, 番号 4, 05.06.2001, p. 293-300.

研究成果: Article

Tanaka, S, Katano, H, Tsukamoto, K, Jin, M, Oikawa, S, Nishihara, H, Sawa, H, Sawada, K, Shimizu, M, Sata, T, Fujioka, Y & Nagashima, K 2001, 'HHV8-negative primary effusion lymphoma of the peritoneal cavity presenting with a distinct immunohistochemical phenotype', Pathology international, 巻. 51, 番号 4, pp. 293-300. https://doi.org/10.1046/j.1440-1827.2001.01189.x
Tanaka, Shinya ; Katano, Harutaka ; Tsukamoto, Kenichi ; Jin, Mooran ; Oikawa, Sumie ; Nishihara, Hiroshi ; Sawa, Hirofumi ; Sawada, Kenichi ; Shimizu, Michio ; Sata, Tetsutaro ; Fujioka, Yasunori ; Nagashima, Kazuo. / HHV8-negative primary effusion lymphoma of the peritoneal cavity presenting with a distinct immunohistochemical phenotype. :: Pathology international. 2001 ; 巻 51, 番号 4. pp. 293-300.
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AU - Tanaka, Shinya

AU - Katano, Harutaka

AU - Tsukamoto, Kenichi

AU - Jin, Mooran

AU - Oikawa, Sumie

AU - Nishihara, Hiroshi

AU - Sawa, Hirofumi

AU - Sawada, Kenichi

AU - Shimizu, Michio

AU - Sata, Tetsutaro

AU - Fujioka, Yasunori

AU - Nagashima, Kazuo

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AB - Primary effusion lymphoma (PEL) has been recognized as a body-cavity-based lymphoma that was originally reported to be associated with human herpes virus 8 (HHV8) infection, and was frequently found in human immunodeficiency virus-positive (HIV) patients. Here we describe an autopsy case of PEL of the peritoneal cavity in an immunocompetent patient. Cytological analysis of tumor cells within ascites revealed immunocytochemical features of keratin positivity and CD45 negativity. At autopsy, the presence of a massive volume of ascites as well as diffuse tumor cell infiltrates within the serosa of the intestine and mesenterium were observed. Tumor cells were morphologically similar to anaplastic large-cell lymphoma, but were immunohistochemically positive for keratin and epithelial membrane antigen (EMA). They also showed no reactivity to representative lymphocyte surface markers including CD45, in addition to being negative for CD30 and p80NPM/ALK. Molecular analysis of the tumor cells revealed monoclonality of the immunoglobulin heavy-chain gene rearrangement which demonstrated a lymphoma of the B-cell lineage. Furthermore, HHV8 was not detected by immunohistochemical analysis, PCR or nested PCR technique. Based on these results, we consider the present case to be an HHV8-negative PEL with keratin and EMA positivity.

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